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wild type wt hela cells  (ATCC)


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    ATCC wild type wt hela cells
    Wild Type Wt Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+wt+hela+cells/HeLa/pm39827287-457-12-16
    Average 99 stars, based on 10447 article reviews
    wild type wt hela cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Identification and structural characterization of small molecule inhibitors of PINK1
    Article Snippet: The IC 50 value and standard deviation were determined in GraphPad. .. Wild type (WT) HeLa cells (ATCC #CCL-2) were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) at 37 °C and 5% CO 2 in a humidified atmosphere. ..

    Stable Transfection:

    Article Title: Highly Specific Protein Identification by Immunoprecipitation–Mass Spectrometry Using Antifouling Microbeads
    Article Snippet: It is also recommended to quickly collect all beads after each incubation step by a pulse spin of a few seconds in an Eppendorf centrifuge. .. Wild-type (WT) HeLa cells (HeLa B-50 from ATCC (CRL-12401)) and HeLa cells stably expressing MBD3-GFP or EED-GFP (kindly provided by Prof. Dr. M. Vermeulen from the Radboud Institute of Molecular Life Sciences (RIMLS)) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% FCS, 100 μg/mL penicillin, and 100 μg/mL streptomycin at 37 °C and 5% CO 2 . ..

    Expressing:

    Article Title: Highly Specific Protein Identification by Immunoprecipitation–Mass Spectrometry Using Antifouling Microbeads
    Article Snippet: It is also recommended to quickly collect all beads after each incubation step by a pulse spin of a few seconds in an Eppendorf centrifuge. .. Wild-type (WT) HeLa cells (HeLa B-50 from ATCC (CRL-12401)) and HeLa cells stably expressing MBD3-GFP or EED-GFP (kindly provided by Prof. Dr. M. Vermeulen from the Radboud Institute of Molecular Life Sciences (RIMLS)) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% FCS, 100 μg/mL penicillin, and 100 μg/mL streptomycin at 37 °C and 5% CO 2 . ..

    Modification:

    Article Title: Highly Specific Protein Identification by Immunoprecipitation–Mass Spectrometry Using Antifouling Microbeads
    Article Snippet: It is also recommended to quickly collect all beads after each incubation step by a pulse spin of a few seconds in an Eppendorf centrifuge. .. Wild-type (WT) HeLa cells (HeLa B-50 from ATCC (CRL-12401)) and HeLa cells stably expressing MBD3-GFP or EED-GFP (kindly provided by Prof. Dr. M. Vermeulen from the Radboud Institute of Molecular Life Sciences (RIMLS)) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% FCS, 100 μg/mL penicillin, and 100 μg/mL streptomycin at 37 °C and 5% CO 2 . ..



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    hela wild type wt cells  (ATCC)
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    INTS6 is required for efficient DNA damage repair. ( A ) Left: Representative images of the clonogenic assay in control and INTS6 knockdown cells. The cells were stained and counted after 10 days of growing. Right: Quantification of left. * P ≤ 0.05, ** P ≤ 0.01. ( B ) MTT assay to show cell viability (%) at indicated time points in siCtrl and siINTS6 cells with or without 2 Gy IR treatment. Error bar = mean ± SD, significance was determined using unpaired Student’s t -test, * P ≤ 0.05. ( C ) Left: Drawing of DR-GFP HR reporter strategy. Right: Bar chart shows the efficiency of HR repair in DR-GFP <t>HeLa</t> reporter cells, as measured by FACS. BRCA1 knockdown was used as the positive control. ****P ≤ 0.0001, ***P ≤ 0.001. ( D ) Top: Representative images of comet assay in siCtrl, siINTS6, siRAD51 and si53BP1 cells. siRAD51 and si53BP1 cells were used as positive controls. IR = 5 Gy. Error bar = 100 μm. Bottom: Quantification of top. **** P ≤ 0.0001, ** P ≤ 0.01. ( E ) Model: INTS6 as part of tetrameric SOSS1 complex binds to DNA:RNA hybrids at DSBs and recruits PP2A to dephosphorylate RNAPII. Depletion of INTS6 results in increased levels of DARTs and DNA:RNA hybrids. INTS6 interacts with SETX and is required for its recruitment to damaged sites. SETX, in turn, resolves DNA:RNA hybrids at DSBs facilitating their INTS6-dependent autoregulation. Image was created with Biorender.
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    Image Search Results


    INTS6 is required for efficient DNA damage repair. ( A ) Left: Representative images of the clonogenic assay in control and INTS6 knockdown cells. The cells were stained and counted after 10 days of growing. Right: Quantification of left. * P ≤ 0.05, ** P ≤ 0.01. ( B ) MTT assay to show cell viability (%) at indicated time points in siCtrl and siINTS6 cells with or without 2 Gy IR treatment. Error bar = mean ± SD, significance was determined using unpaired Student’s t -test, * P ≤ 0.05. ( C ) Left: Drawing of DR-GFP HR reporter strategy. Right: Bar chart shows the efficiency of HR repair in DR-GFP HeLa reporter cells, as measured by FACS. BRCA1 knockdown was used as the positive control. ****P ≤ 0.0001, ***P ≤ 0.001. ( D ) Top: Representative images of comet assay in siCtrl, siINTS6, siRAD51 and si53BP1 cells. siRAD51 and si53BP1 cells were used as positive controls. IR = 5 Gy. Error bar = 100 μm. Bottom: Quantification of top. **** P ≤ 0.0001, ** P ≤ 0.01. ( E ) Model: INTS6 as part of tetrameric SOSS1 complex binds to DNA:RNA hybrids at DSBs and recruits PP2A to dephosphorylate RNAPII. Depletion of INTS6 results in increased levels of DARTs and DNA:RNA hybrids. INTS6 interacts with SETX and is required for its recruitment to damaged sites. SETX, in turn, resolves DNA:RNA hybrids at DSBs facilitating their INTS6-dependent autoregulation. Image was created with Biorender.

    Journal: Nucleic Acids Research

    Article Title: Tetrameric INTS6-SOSS1 complex facilitates DNA:RNA hybrid autoregulation at double-strand breaks

    doi: 10.1093/nar/gkae937

    Figure Lengend Snippet: INTS6 is required for efficient DNA damage repair. ( A ) Left: Representative images of the clonogenic assay in control and INTS6 knockdown cells. The cells were stained and counted after 10 days of growing. Right: Quantification of left. * P ≤ 0.05, ** P ≤ 0.01. ( B ) MTT assay to show cell viability (%) at indicated time points in siCtrl and siINTS6 cells with or without 2 Gy IR treatment. Error bar = mean ± SD, significance was determined using unpaired Student’s t -test, * P ≤ 0.05. ( C ) Left: Drawing of DR-GFP HR reporter strategy. Right: Bar chart shows the efficiency of HR repair in DR-GFP HeLa reporter cells, as measured by FACS. BRCA1 knockdown was used as the positive control. ****P ≤ 0.0001, ***P ≤ 0.001. ( D ) Top: Representative images of comet assay in siCtrl, siINTS6, siRAD51 and si53BP1 cells. siRAD51 and si53BP1 cells were used as positive controls. IR = 5 Gy. Error bar = 100 μm. Bottom: Quantification of top. **** P ≤ 0.0001, ** P ≤ 0.01. ( E ) Model: INTS6 as part of tetrameric SOSS1 complex binds to DNA:RNA hybrids at DSBs and recruits PP2A to dephosphorylate RNAPII. Depletion of INTS6 results in increased levels of DARTs and DNA:RNA hybrids. INTS6 interacts with SETX and is required for its recruitment to damaged sites. SETX, in turn, resolves DNA:RNA hybrids at DSBs facilitating their INTS6-dependent autoregulation. Image was created with Biorender.

    Article Snippet: HeLa wild-type (WT) cells were obtained from ATCC.

    Techniques: Clonogenic Assay, Control, Knockdown, Staining, MTT Assay, Positive Control, Single Cell Gel Electrophoresis